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rabbit polyclonal anti bid fl 195 antibody  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rabbit polyclonal anti bid fl 195 antibody
    The majority of cell deaths occurred after prolonged mitotic arrest. a Cell extracts were prepared from HeLa cells after 16 h of the different drug treatments as described previously. The western blot membranes were probed with a rabbit <t>polyclonal</t> anti-phospho-histone H3 (S-10) antibody (Millipore, #06-570) (1:500 dilutions), and the actin protein bands acted as the loading control. b Quantitative comparison of the phospho-histone 3 S-10 bands. Results produced from three independent experiments. c The confocal time-lapse images showing an example of a normal/unperturbed HeLa H2B-GFP cell undergoing the cell cycle in mitosis. d An example of a HeLa H2B-GFP cell undergoing apoptosis after prolonged mitotic arrest by 60 ng/ml nocodazole or 60 ng/ml nocodazole + 50 μM M2I-1. The white arrowhead indicates the time point when the cytoplasmic membrane (DIC grey images) of the cell starts shrinking and rounding up with condensed chromosomes (green). The diamond heads indicate the time point that the cytoplasmic membrane began blebbing. The chromosomes displaying fragmentation and over-condensation are highlighted by white dashed arrows and normal arrows respectively. A typical apoptotic-body like morphology is highlighted with the asterisk. e An example of a HeLa H2B-GFP cell undergoing apoptosis at a prophase-like stage induced by treatment with 60 ng/ml nocodazole + 50 μM M2I-1. The white arrowhead highlights the time point where there is clear cell body shrinkage, dash arrows highlight the fragmenting chromosomes and normal black arrows indicate the intact cytoplasmic membrane during chromosome fragmentation. Asterisks highlight the formation of a typical apoptotic-body like morphology. The green histone 2B-GFP highlights the chromosomal morphologies and the DIC images in grey indicate the cellular boundaries and the cytoplasmic membrane. The timing of the images is indicated
    Rabbit Polyclonal Anti Bid Fl 195 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 451 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti bid fl 195 antibody/product/Santa Cruz Biotechnology
    Average 95 stars, based on 451 article reviews
    rabbit polyclonal anti bid fl 195 antibody - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "M2I-1 disrupts the in vivo interaction between CDC20 and MAD2 and increases the sensitivities of cancer cell lines to anti-mitotic drugs via MCL-1s"

    Article Title: M2I-1 disrupts the in vivo interaction between CDC20 and MAD2 and increases the sensitivities of cancer cell lines to anti-mitotic drugs via MCL-1s

    Journal: Cell Division

    doi: 10.1186/s13008-019-0049-5

    The majority of cell deaths occurred after prolonged mitotic arrest. a Cell extracts were prepared from HeLa cells after 16 h of the different drug treatments as described previously. The western blot membranes were probed with a rabbit polyclonal anti-phospho-histone H3 (S-10) antibody (Millipore, #06-570) (1:500 dilutions), and the actin protein bands acted as the loading control. b Quantitative comparison of the phospho-histone 3 S-10 bands. Results produced from three independent experiments. c The confocal time-lapse images showing an example of a normal/unperturbed HeLa H2B-GFP cell undergoing the cell cycle in mitosis. d An example of a HeLa H2B-GFP cell undergoing apoptosis after prolonged mitotic arrest by 60 ng/ml nocodazole or 60 ng/ml nocodazole + 50 μM M2I-1. The white arrowhead indicates the time point when the cytoplasmic membrane (DIC grey images) of the cell starts shrinking and rounding up with condensed chromosomes (green). The diamond heads indicate the time point that the cytoplasmic membrane began blebbing. The chromosomes displaying fragmentation and over-condensation are highlighted by white dashed arrows and normal arrows respectively. A typical apoptotic-body like morphology is highlighted with the asterisk. e An example of a HeLa H2B-GFP cell undergoing apoptosis at a prophase-like stage induced by treatment with 60 ng/ml nocodazole + 50 μM M2I-1. The white arrowhead highlights the time point where there is clear cell body shrinkage, dash arrows highlight the fragmenting chromosomes and normal black arrows indicate the intact cytoplasmic membrane during chromosome fragmentation. Asterisks highlight the formation of a typical apoptotic-body like morphology. The green histone 2B-GFP highlights the chromosomal morphologies and the DIC images in grey indicate the cellular boundaries and the cytoplasmic membrane. The timing of the images is indicated
    Figure Legend Snippet: The majority of cell deaths occurred after prolonged mitotic arrest. a Cell extracts were prepared from HeLa cells after 16 h of the different drug treatments as described previously. The western blot membranes were probed with a rabbit polyclonal anti-phospho-histone H3 (S-10) antibody (Millipore, #06-570) (1:500 dilutions), and the actin protein bands acted as the loading control. b Quantitative comparison of the phospho-histone 3 S-10 bands. Results produced from three independent experiments. c The confocal time-lapse images showing an example of a normal/unperturbed HeLa H2B-GFP cell undergoing the cell cycle in mitosis. d An example of a HeLa H2B-GFP cell undergoing apoptosis after prolonged mitotic arrest by 60 ng/ml nocodazole or 60 ng/ml nocodazole + 50 μM M2I-1. The white arrowhead indicates the time point when the cytoplasmic membrane (DIC grey images) of the cell starts shrinking and rounding up with condensed chromosomes (green). The diamond heads indicate the time point that the cytoplasmic membrane began blebbing. The chromosomes displaying fragmentation and over-condensation are highlighted by white dashed arrows and normal arrows respectively. A typical apoptotic-body like morphology is highlighted with the asterisk. e An example of a HeLa H2B-GFP cell undergoing apoptosis at a prophase-like stage induced by treatment with 60 ng/ml nocodazole + 50 μM M2I-1. The white arrowhead highlights the time point where there is clear cell body shrinkage, dash arrows highlight the fragmenting chromosomes and normal black arrows indicate the intact cytoplasmic membrane during chromosome fragmentation. Asterisks highlight the formation of a typical apoptotic-body like morphology. The green histone 2B-GFP highlights the chromosomal morphologies and the DIC images in grey indicate the cellular boundaries and the cytoplasmic membrane. The timing of the images is indicated

    Techniques Used: Western Blot, Control, Comparison, Produced, Membrane

    The regulation of the stability of MCL-1, cyclin B1, and other pro- or anti-apoptotic proteins in HeLa and MCF-7 cells. a Western blots of cell extracts prepared from HeLa cells after treatment with 0.5% DMSO, 50 μM M2I-1, 60 ng/ml nocodazole, and 60 ng/ml nocodazole + 50 μM M2I-1 respectively and probed with a rabbit polyclonal anti-cyclin B1 antibody (H-433) antibody (sc-7520); a mouse monoclonal anti-actin (AC-15) antibody (ab6276) was used as the loading control. b – f Western blot results showing the expression levels of the pro-apoptotic proteins BIM, BID, PUMA, NOXA, and MCL-1s in HeLa cells. Cell extracts were prepared from HeLa cells after treatment with nocodazole alone or nocodazole + M2I-1 as described before. Actin bands were used as the loading controls. MCF-7 cells were treated using the same conditions as described in Fig. e apart from the determination of the chromosomal morphologies, which was highlighted by staining with 0.2 μ M SiR-DNA. Digital images were taken after 20 h incubation for quantification of the mitotic ( g ), apoptotic ( h ), and slippage indices ( i ). n: The number of the parallel experiments and the total cell numbers used for quantification. P value * < 0.026, 0.029, and 0.036 respectively
    Figure Legend Snippet: The regulation of the stability of MCL-1, cyclin B1, and other pro- or anti-apoptotic proteins in HeLa and MCF-7 cells. a Western blots of cell extracts prepared from HeLa cells after treatment with 0.5% DMSO, 50 μM M2I-1, 60 ng/ml nocodazole, and 60 ng/ml nocodazole + 50 μM M2I-1 respectively and probed with a rabbit polyclonal anti-cyclin B1 antibody (H-433) antibody (sc-7520); a mouse monoclonal anti-actin (AC-15) antibody (ab6276) was used as the loading control. b – f Western blot results showing the expression levels of the pro-apoptotic proteins BIM, BID, PUMA, NOXA, and MCL-1s in HeLa cells. Cell extracts were prepared from HeLa cells after treatment with nocodazole alone or nocodazole + M2I-1 as described before. Actin bands were used as the loading controls. MCF-7 cells were treated using the same conditions as described in Fig. e apart from the determination of the chromosomal morphologies, which was highlighted by staining with 0.2 μ M SiR-DNA. Digital images were taken after 20 h incubation for quantification of the mitotic ( g ), apoptotic ( h ), and slippage indices ( i ). n: The number of the parallel experiments and the total cell numbers used for quantification. P value * < 0.026, 0.029, and 0.036 respectively

    Techniques Used: Western Blot, Control, Expressing, Staining, Incubation



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    The majority of cell deaths occurred after prolonged mitotic arrest. a Cell extracts were prepared from HeLa cells after 16 h of the different drug treatments as described previously. The western blot membranes were probed with a rabbit <t>polyclonal</t> anti-phospho-histone H3 (S-10) antibody (Millipore, #06-570) (1:500 dilutions), and the actin protein bands acted as the loading control. b Quantitative comparison of the phospho-histone 3 S-10 bands. Results produced from three independent experiments. c The confocal time-lapse images showing an example of a normal/unperturbed HeLa H2B-GFP cell undergoing the cell cycle in mitosis. d An example of a HeLa H2B-GFP cell undergoing apoptosis after prolonged mitotic arrest by 60 ng/ml nocodazole or 60 ng/ml nocodazole + 50 μM M2I-1. The white arrowhead indicates the time point when the cytoplasmic membrane (DIC grey images) of the cell starts shrinking and rounding up with condensed chromosomes (green). The diamond heads indicate the time point that the cytoplasmic membrane began blebbing. The chromosomes displaying fragmentation and over-condensation are highlighted by white dashed arrows and normal arrows respectively. A typical apoptotic-body like morphology is highlighted with the asterisk. e An example of a HeLa H2B-GFP cell undergoing apoptosis at a prophase-like stage induced by treatment with 60 ng/ml nocodazole + 50 μM M2I-1. The white arrowhead highlights the time point where there is clear cell body shrinkage, dash arrows highlight the fragmenting chromosomes and normal black arrows indicate the intact cytoplasmic membrane during chromosome fragmentation. Asterisks highlight the formation of a typical apoptotic-body like morphology. The green histone 2B-GFP highlights the chromosomal morphologies and the DIC images in grey indicate the cellular boundaries and the cytoplasmic membrane. The timing of the images is indicated
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    Image Search Results


    The majority of cell deaths occurred after prolonged mitotic arrest. a Cell extracts were prepared from HeLa cells after 16 h of the different drug treatments as described previously. The western blot membranes were probed with a rabbit polyclonal anti-phospho-histone H3 (S-10) antibody (Millipore, #06-570) (1:500 dilutions), and the actin protein bands acted as the loading control. b Quantitative comparison of the phospho-histone 3 S-10 bands. Results produced from three independent experiments. c The confocal time-lapse images showing an example of a normal/unperturbed HeLa H2B-GFP cell undergoing the cell cycle in mitosis. d An example of a HeLa H2B-GFP cell undergoing apoptosis after prolonged mitotic arrest by 60 ng/ml nocodazole or 60 ng/ml nocodazole + 50 μM M2I-1. The white arrowhead indicates the time point when the cytoplasmic membrane (DIC grey images) of the cell starts shrinking and rounding up with condensed chromosomes (green). The diamond heads indicate the time point that the cytoplasmic membrane began blebbing. The chromosomes displaying fragmentation and over-condensation are highlighted by white dashed arrows and normal arrows respectively. A typical apoptotic-body like morphology is highlighted with the asterisk. e An example of a HeLa H2B-GFP cell undergoing apoptosis at a prophase-like stage induced by treatment with 60 ng/ml nocodazole + 50 μM M2I-1. The white arrowhead highlights the time point where there is clear cell body shrinkage, dash arrows highlight the fragmenting chromosomes and normal black arrows indicate the intact cytoplasmic membrane during chromosome fragmentation. Asterisks highlight the formation of a typical apoptotic-body like morphology. The green histone 2B-GFP highlights the chromosomal morphologies and the DIC images in grey indicate the cellular boundaries and the cytoplasmic membrane. The timing of the images is indicated

    Journal: Cell Division

    Article Title: M2I-1 disrupts the in vivo interaction between CDC20 and MAD2 and increases the sensitivities of cancer cell lines to anti-mitotic drugs via MCL-1s

    doi: 10.1186/s13008-019-0049-5

    Figure Lengend Snippet: The majority of cell deaths occurred after prolonged mitotic arrest. a Cell extracts were prepared from HeLa cells after 16 h of the different drug treatments as described previously. The western blot membranes were probed with a rabbit polyclonal anti-phospho-histone H3 (S-10) antibody (Millipore, #06-570) (1:500 dilutions), and the actin protein bands acted as the loading control. b Quantitative comparison of the phospho-histone 3 S-10 bands. Results produced from three independent experiments. c The confocal time-lapse images showing an example of a normal/unperturbed HeLa H2B-GFP cell undergoing the cell cycle in mitosis. d An example of a HeLa H2B-GFP cell undergoing apoptosis after prolonged mitotic arrest by 60 ng/ml nocodazole or 60 ng/ml nocodazole + 50 μM M2I-1. The white arrowhead indicates the time point when the cytoplasmic membrane (DIC grey images) of the cell starts shrinking and rounding up with condensed chromosomes (green). The diamond heads indicate the time point that the cytoplasmic membrane began blebbing. The chromosomes displaying fragmentation and over-condensation are highlighted by white dashed arrows and normal arrows respectively. A typical apoptotic-body like morphology is highlighted with the asterisk. e An example of a HeLa H2B-GFP cell undergoing apoptosis at a prophase-like stage induced by treatment with 60 ng/ml nocodazole + 50 μM M2I-1. The white arrowhead highlights the time point where there is clear cell body shrinkage, dash arrows highlight the fragmenting chromosomes and normal black arrows indicate the intact cytoplasmic membrane during chromosome fragmentation. Asterisks highlight the formation of a typical apoptotic-body like morphology. The green histone 2B-GFP highlights the chromosomal morphologies and the DIC images in grey indicate the cellular boundaries and the cytoplasmic membrane. The timing of the images is indicated

    Article Snippet: Primary antibodies used in this project are Rabbit polyclonal anti-CDC20 antibody (Abcam, ab26483); mouse monoclonal anti-p55 CDC (E-7) (Santa Cruz Biotech, sc-13162); rabbit polyclonal anti-full length MAD2 (Convance, PRB-452C); mouse monoclonal anti-cyclin B1 (GNS) (Santa Cruz, sc-245); mouse monoclonal anti-cyclin A (B-8) (Santa Cruz, sc-271682); mouse monoclonal anti-actin antibody (Abcam, ab6276); mouse monoclonal anti-GADPH antibody (Thermo Fisher Scientific, MA5-15738); rabbit polyclonal anti-caspase-3 antibody (Abcam, ab32351); rabbit polyclonal anti-phospho-histone 3 (S-10) antibody (Millipore, #06-570); and rabbit polyclonal anti-GFP antibody [Santa Cruz, sc-8334 (GP-FL)]; rabbit polyclonal anti-γ-H2AX (S-139) antibody (Abcam, ab-2893); GFP-Trap A geta-20 (ChromoTek, 70112001A); rabbit polyclonal anti-Mcl-1 (S-19) antibody (Santa Cruz, sc-819); rabbit polyclonal anti-pericentrin 1&2 antibody (Abcam, ab4448); rabbit polyclonal anti-BID (FL-195) antibody (Santa Cruz, sc-11423); mouse monoclonal anti-BIM (H-5) antibody (Santa Cruz, sc-374358); rabbit polyclonal anti-NOXA (FL-54) antibody (Santa Cruz, sc-30209) and monoclonal anti-PUMA (G-3) antibody (Santa Cruz, sc-374223); rabbit monoclonal anti-human PARP-1 antibody (46D11) (Cell Signaling Technology).

    Techniques: Western Blot, Control, Comparison, Produced, Membrane

    The regulation of the stability of MCL-1, cyclin B1, and other pro- or anti-apoptotic proteins in HeLa and MCF-7 cells. a Western blots of cell extracts prepared from HeLa cells after treatment with 0.5% DMSO, 50 μM M2I-1, 60 ng/ml nocodazole, and 60 ng/ml nocodazole + 50 μM M2I-1 respectively and probed with a rabbit polyclonal anti-cyclin B1 antibody (H-433) antibody (sc-7520); a mouse monoclonal anti-actin (AC-15) antibody (ab6276) was used as the loading control. b – f Western blot results showing the expression levels of the pro-apoptotic proteins BIM, BID, PUMA, NOXA, and MCL-1s in HeLa cells. Cell extracts were prepared from HeLa cells after treatment with nocodazole alone or nocodazole + M2I-1 as described before. Actin bands were used as the loading controls. MCF-7 cells were treated using the same conditions as described in Fig. e apart from the determination of the chromosomal morphologies, which was highlighted by staining with 0.2 μ M SiR-DNA. Digital images were taken after 20 h incubation for quantification of the mitotic ( g ), apoptotic ( h ), and slippage indices ( i ). n: The number of the parallel experiments and the total cell numbers used for quantification. P value * < 0.026, 0.029, and 0.036 respectively

    Journal: Cell Division

    Article Title: M2I-1 disrupts the in vivo interaction between CDC20 and MAD2 and increases the sensitivities of cancer cell lines to anti-mitotic drugs via MCL-1s

    doi: 10.1186/s13008-019-0049-5

    Figure Lengend Snippet: The regulation of the stability of MCL-1, cyclin B1, and other pro- or anti-apoptotic proteins in HeLa and MCF-7 cells. a Western blots of cell extracts prepared from HeLa cells after treatment with 0.5% DMSO, 50 μM M2I-1, 60 ng/ml nocodazole, and 60 ng/ml nocodazole + 50 μM M2I-1 respectively and probed with a rabbit polyclonal anti-cyclin B1 antibody (H-433) antibody (sc-7520); a mouse monoclonal anti-actin (AC-15) antibody (ab6276) was used as the loading control. b – f Western blot results showing the expression levels of the pro-apoptotic proteins BIM, BID, PUMA, NOXA, and MCL-1s in HeLa cells. Cell extracts were prepared from HeLa cells after treatment with nocodazole alone or nocodazole + M2I-1 as described before. Actin bands were used as the loading controls. MCF-7 cells were treated using the same conditions as described in Fig. e apart from the determination of the chromosomal morphologies, which was highlighted by staining with 0.2 μ M SiR-DNA. Digital images were taken after 20 h incubation for quantification of the mitotic ( g ), apoptotic ( h ), and slippage indices ( i ). n: The number of the parallel experiments and the total cell numbers used for quantification. P value * < 0.026, 0.029, and 0.036 respectively

    Article Snippet: Primary antibodies used in this project are Rabbit polyclonal anti-CDC20 antibody (Abcam, ab26483); mouse monoclonal anti-p55 CDC (E-7) (Santa Cruz Biotech, sc-13162); rabbit polyclonal anti-full length MAD2 (Convance, PRB-452C); mouse monoclonal anti-cyclin B1 (GNS) (Santa Cruz, sc-245); mouse monoclonal anti-cyclin A (B-8) (Santa Cruz, sc-271682); mouse monoclonal anti-actin antibody (Abcam, ab6276); mouse monoclonal anti-GADPH antibody (Thermo Fisher Scientific, MA5-15738); rabbit polyclonal anti-caspase-3 antibody (Abcam, ab32351); rabbit polyclonal anti-phospho-histone 3 (S-10) antibody (Millipore, #06-570); and rabbit polyclonal anti-GFP antibody [Santa Cruz, sc-8334 (GP-FL)]; rabbit polyclonal anti-γ-H2AX (S-139) antibody (Abcam, ab-2893); GFP-Trap A geta-20 (ChromoTek, 70112001A); rabbit polyclonal anti-Mcl-1 (S-19) antibody (Santa Cruz, sc-819); rabbit polyclonal anti-pericentrin 1&2 antibody (Abcam, ab4448); rabbit polyclonal anti-BID (FL-195) antibody (Santa Cruz, sc-11423); mouse monoclonal anti-BIM (H-5) antibody (Santa Cruz, sc-374358); rabbit polyclonal anti-NOXA (FL-54) antibody (Santa Cruz, sc-30209) and monoclonal anti-PUMA (G-3) antibody (Santa Cruz, sc-374223); rabbit monoclonal anti-human PARP-1 antibody (46D11) (Cell Signaling Technology).

    Techniques: Western Blot, Control, Expressing, Staining, Incubation